舒城县干汊河镇农村集体“三资”管理现状与建议

阐述了舒城县干汊河镇农村集体"三资"管理取得的成效,指出了管理过程中存在的问题,并提出了加强农村集体"三资"管理的建议。     

黑土长缓坡地形与横垄对土壤有机碳空间分异的交互作用

横坡垄作对坡耕地土壤有机碳（soil organic carbon,SOC）流失有一定的阻控作用,但黑土区特有长而缓的地形与横垄对坡耕地SOC空间分异会产生交互作用,而这种交互作用引发的SOC流失风险没引起足够的重视。该研究以典型黑土区黑龙江省黑河市北安分局红星农场为研究区域,2022年在横坡垄作与顺坡水线方向上共布设25个采样点,采用地理探测器模型、单因素方差分析（one-way ANOVA）和Pearson相关性分析,探讨土壤有机碳的空间分异及其交互作用。结果表明,横坡垄作方向上垄沟土壤有机碳含量从坡顶到水线呈现逐渐增大的变化趋势;在垄台从坡顶到水线呈现先增大后减小的变化趋势。顺坡水线方向,土壤有机碳含量在垄沟呈现从上坡到下坡增大的变化趋势;在垄台呈现先增大后减小的变化趋势。由于断垄产生水线,顺坡土壤有机碳含量上坡与下坡仍有显著差异（P＜0.05）。Pearson相关性分析表明, 有机碳与可蚀性K因子呈显著负相关（垄沟和垄台相关系数分别为–0.228和–0.238,P＜0.05）,与碳循环相关的β-葡萄糖苷酶和微生物生物量碳在垄沟呈极显著正相关（相关系数为0.398和0.676,P<0.01）。地理探测器分析表明,顺坡水线对土壤有机碳空间分异的影响最大,其对垄沟和垄台SOC的解释力分别达到61%和52%以上;顺坡水线与其他因子的交互作用共同增强了对土壤有机碳的解释力,尤其是顺坡水线与高程的交互作用最为明显。黑土区坡耕地土壤有机碳空间分异主要受顺坡水线与高程的交互作用,横坡垄作虽然能够拦截径流,但由于长缓坡地形影响产生的断垄会加剧土壤侵蚀诱发的有机碳流失。因此,黑土坡耕地治理需要同时考虑横垄与地形的共同影响,从而实现防蚀的优化效果。     

青海东部农区引进燕麦品种各器官C、N、P生态化学计量学特征变化研究

以4个引种燕麦(Avena sativa)为典型代表,对其不同生育时期根、茎、叶中的C、N、P含量及其化学计量学特征进行测定,探讨不同生育时期燕麦C、N、P元素含量及其化学计量比的变化规律,为燕麦饲草的科学收获提供理论依据。结果表明：燕麦全株C、N、P含量分别为322.30～333.97、17.42～75.62、2.74～5.42 mg·g^-1,燕麦根C、N、P含量分别为298.42～317.92、11.47～73.71、2.82～3.42 mg·g^-1,燕麦茎C、N、P含量分别为311.25～338.86、10.15～75.16、2.44～5.06 mg·g^-1,燕麦叶C、N、P含量分别为330.80～372.47、30.64～113.80、2.59～8.65 mg·g^-1;各器官间C、N、P含量表现为叶>茎>根。此外,燕麦各器官C、N、P含量的积累过程具有一定季节特征,C含量积累过程受生育时期影响较小,表现出较强的稳定性;而N和P含量的积累过程受生育时期影响较大,其在拔节期～抽穗期均...     

县域耕地土壤养分时空变异特征研究

土壤养分空间变异研究是精准农业实施的前提,对提高粮食作物单位面积产量和保护农业生态环境具有重要作用。为掌握陕西渭北旱塬区土壤养分时空变异规律,综合空间自相关和重心模型,分析1980—2007年、2007—2017年白水县耕地土壤有机质(SOM)、速效磷(AP)和速效钾(AK)的时空变异特征。结果表明：(1)研究区土壤SOM的平均含量分别增加了1.29、3.22 g·kg^-1,AP的平均含量分别增加了6.89、24.45 mg·kg^-1,仅有AK的平均含量呈现出先减少后增加的趋势。(2)三期耕地土壤养分的变异系数范围为30.2%～90.8%,属于中等变异。1980—2017年,耕地土壤SOM、AP和AK的全局Moran’s I指数均降低,空间结构性减弱,随机因素作用增强。(3)土壤SOM、AP和AK含量均有不同程度的增加,土壤SOM含量总体呈北低南高分布,土壤AP、AK含量分布情况较为一致,呈东高西低。(4)土壤SOM含量重心在东南方向发生偏移,AP、AK含量重心在东北方向发生偏移。土壤养分标准差椭圆空间分布格局同研究区分布方向一致,转角呈现“...     

Immunization of BALB/c mice with Brucella abortus 2308ΔwbkA confers protection against wild-type infection

Brucellosis is a zoonotic disease that causes animal and human diseases. Vaccination is a major measure for prevention of brucellosis, but it is currently not possible to distinguish vaccinated animals from those that have been naturally infected. Therefore, in this study, we constructed the Brucella (B.) abortus 2380 wbkA mutant (2308ΔwbkA) and evaluated its virulence. The survival of 2308ΔwbkA was attenuated in murine macrophage (RAW 264.7) and BALB/c mice, and it induced high protective immunity in mice. The wbkA mutant elicited an anti-Brucella-specific immunoglobulin G response and induced the secretion of gamma interferon. Antibodies to 2308ΔwbkA could be detected in sera from mice, implying the potential for use of this protein as a diagnostic antigen. The WbkA antigen would allow serological differentiation between infected and vaccinated animals. These results suggest that 2308ΔwbkA is a potential attenuated vaccine against 16M. This vaccine will be further evaluated in sheep.     

Variations in the morphology of Rhizomucor pusillus in granulomatous lesions of a Magellanic penguin (Spheniscus magellanicus)

This report presents a new case of mucormycosis encountered in penguin characterized by morphological variation of hyphae and presence of sporangia with numerous sporangiospores. A 4.5-year-old Magellanic penguin (Spheniscus magellanicus) died after exhibiting anorexia, poor nutritional condition and dyspnea. Multiple nodular lesions were observed in the thoracic and abdominal regions. Histopathologically, hyphae of various sizes were seen in the lungs, air sac and nodular lesions. Myriad sporangiospores and several sporangia were observed in/around the bronchi or parabronchi. The very narrow and short hyphae in the nodules were not consistent with the characteristics of Mucorales. However, for most hyphae, including those in the nodules, sporangiospores and sporangia, immunohistochemistry revealed Mucorales-positive reactions. In addition, these fungi were identified as Rhizomucor pusillus by gene analysis.     

Phenotypic and genotypic characterization of canine pyoderma isolates of Staphylococcus pseudintermedius for biofilm formation

Biofilm-forming ability is increasingly being recognized as an important virulence factor in several Staphylococcus species. This study evaluated the biofilm-forming ability of sixty canine derived clinical isolates of S. pseudintermedius, using three phenotypic methods, microtiter plate test (MtP), Congo red agar method (CRA) and tube adherence test, and the presence and impact of biofilm-associated genes (icaA and icaD). The results showed that icaA and icaD genes were detected concomitantly in 55 (91.7%) of 60 isolates. A majority (88.3%) of the strains screened had matching results by the tube adherence test, MtP and PCR analysis. Better agreement (95%) was found between the PCR-based analysis and the CRA. Results of the icaA and icaD gene PCRs showed good agreement with CRA results, with a kappa of 0.7. Comparing the phenotypic methods, the statistical analysis showed that the agreement among the phenotypical tests using categorical data was generally good. Considering two classes (biofilm producer and biofilm non-producer), the percentage of matching results between the CRA method and the tube adherence test and between the CRA method and the MtP was 93.3%. A concordance of 100% was revealed between the MtP and the tube adherence test. The results indicate a high prevalence of the ica genes within S. pseudintermedius isolates, and their presence is associated with in vitro formation of a biofilm. A combination of phenotypic and genotypic tests is recommended for investigating biofilm formation in S. pseudintermedius.     

Isolation and antimicrobial susceptibility of Plesiomonas shigelloides from great cormorants (Phalacrocorax carbo hanedae) in Gifu and Shiga Prefectures,Japan

Plesiomonas shigelloides is a causal agent of gastroenteritis, sepsis and meningitis in humans. We examined the prevalence of P. shigelloides among great cormorants (Phalacrocorax carbo hanedae) in Japan and the antimicrobial susceptibility of isolates. P. shigelloides was isolated from 33 (47.8%) of 69 fecal samples from great cormorants in 2014. All 33 isolates were subjected to antimicrobial susceptibility testing using broth microdilution methods, which showed resistance to ampicillin (31 isolates, 93.9%), tetracycline (two isolates, 6.1%) and trimethoprim (one isolate, 3.0%). The high prevalence of P. shigelloides in the great cormorants implicates the possible microbiological risk to public health.